Illumina introduces a very fast and accurate resequencing workflow that begins with BCL or FASTQ files and produces BAM and VCF files in just over 7 hours for a 30x genome on commodity comput- ing hardware. This workflow provides a four-to seven-fold speed increase over currently available methods and enables a sample to variant workflow of less than 40 hours, using PCR-free library prepa- ration and sequencing with the HiSeq® 2500 System.
The core algorithms are the Isaac Genome Alignment and the Isaac Variant Caller software’. The workflow is available in BaseSpace”, through Rapid Track Services, and on any computer meeting the recommended specifications, including the IlluminaCompute Systems, under the Illumina Open-Source Software License.
