Formalin-fixed, paraffin-embedded (FFPE) human tissues are a valuable source of material for molecular analyses and clinical studies. Various processes and protocols now exist for the extraction and purification of nucleic acids from FFPE samples. However, the assays used to evaluate DNA and RNA have evolved from simple monoplex PCR to higher plexity products. Therefore, the quality and amount of nucleic acid extracted from FFPE material has become more critical to the success of these assays.
This white paper provides a review of FFPE DNA extraction and qualifications methods as they apply to higher plexity assays in next-generation sequencing analysis. In particular, it will focus on the use of FFPE samples as starting material for library preparation with the TruSight Tumor 26 panel for sequencing on the Illumina MiSeq* desktop sequencer.
